For the taeGut3.2.4 assembly, the predicted gene set contains 18,447 gene models, of which 17,475 are protein coding genes. The gene set was built using a modified version of the standard EnsEMBL genebuild pipeline. Unusually, some genes relying on EST evidence were included in the gene set. The evidence used to construct the gene set included alignments of EnsEMBL chicken proteins, alignments of other avian proteins and mammal protein alignments, in addition to zebra finch evidence.